不同培养条件对禽源沙门菌菌毛基因表达的影响

doi:10.11843/j.issn.0366-6964.2014.01.016

摘要/Abstract

摘要：

旨在研究不同血清型禽源沙门菌菌毛基因分布特征及不同培养条件下菌毛基因的表达差异。通过PCR方法对鸡白痢沙门菌、鸡伤寒沙门菌、鼠伤寒沙门菌和肠炎沙门菌的17种沙门菌菌毛操纵子基因进行检测。同时，应用RT-PCR方法对肉汤静置、肉汤震荡与平板静置3种培养条件下菌毛基因的表达情况进行检测。研究结果显示，被检测的4种血清型禽源沙门菌菌株的菌毛基因型各不相同，其中10种菌毛基因为保守基因，而其它7种存在差异性分布。RT-PCR检测发现，不同血清型菌株在3种培养条件下菌毛基因的表达存在一定差异，其中鸡白痢沙门菌和肠炎沙门菌在肉汤静置培养条件下表达的菌毛基因数量显著高于其它2种培养条件（P<0.05），而鸡伤寒沙门菌和鼠伤寒沙门菌在3种培养条件下表达的菌毛基因数量差异较小（P>0.05）。结果提示：4种血清型禽源沙门菌菌株具有不同的菌毛基因类型，鸡白痢沙门菌与肠炎沙门菌的菌毛基因表达数量与其培养方式存在相关性。

Abstract:

The objective of the present study was to investigate the distribution of fimbrial genes in some avian Salmonella serotypes and the expression differences of fimbrial genes under different bacterial culture conditions.Seventeen kinds of fimbrial operon genes in Salmonella pullorum，Salmonella gallinarum，Salmonella typhimurium and Salmonella enteritidis were detected by PCR，and expression of fimbrial genes in the above Salmonella strains under three different culture conditions which include static broth，shaken broth and static plate were detected by
RT-PCR.Results showed that the four Salmonella serotypes had different fimbrial genotypes，10 of which were conservative，and the distribution of the other 7 fimbrial genes was different.RT-PCR detection results showed that the expression of fimbrial genes in different
serotypes cultured under three conditions had some differences，as the expression of fimbrial genes in Salmonella pullorum and Salmonella enteritidis cultured on static broth was more than that of others (P<0.05)，while the difference in Salmonella gallinarum and Salmonella
typhimurium was not significant (P>0.05).The results revealed that the four Salmonella serotypes had different fimbrial genotypes and the bacterial culture condition significantly impacted the expression of fimbrial genes in Salmonella pullorum and Salmonella enteritidis.

中图分类号:

S852.612

龚建森，王鑫，韩先干，朱春红，俞燕，刘学贤，徐步. 不同培养条件对禽源沙门菌菌毛基因表达的影响[J]. 畜牧兽医学报, 2014, 45(1): 116-122.

GONG Jian-sen，WANG Xin，HAN Xian-gan，ZHU Chun-hong，YU Yan，LIU Xue-xian，XU Bu. Effects of Different Bacterial Culture Condition on the Expression of Avian Salmonella Fimbrial Genes[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(1): 116-122.

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